Juang R S, Wu F C, Tseng R L
Department of Chemical Engineering, Yuan Ze University, Chung-Li, Taiwan.
Bioresour Technol. 2001 Dec;80(3):187-93. doi: 10.1016/s0960-8524(01)00090-6.
The equilibrium and kinetics of adsorption of reactive dye RR222 and Cu2+, and the activity of immobilization of acid phosphatase, on highly swollen chitosan beads were examined at 30 degrees C. The chitosan was prepared from shrimp shell wastes and was cross-linked with different dosages of glutaraldehyde or glyoxal (100-80,000 mg/l). It was shown that the amounts of solute adsorption and the immobilization capacity of acid phosphatase on cross-linked chitosan beads were substantially affected by their degree of cross-linking. The cross-linking rate of chitosan with glutaraldehyde could be described by a pseudo-second-order equation and the cross-linking equilibrium by the Freundlich equation. This provided an experimental method to control the degree of cross-linking of chitosan beads. Finally, the activity and lifetime of the immobilized enzyme were measured to evaluate the application potential.
在30℃下研究了活性染料RR222和Cu2+在高度溶胀的壳聚糖珠上的吸附平衡和动力学，以及酸性磷酸酶的固定化活性。壳聚糖由虾壳废料制备，并与不同剂量的戊二醛或乙二醛（100 - 80000mg/L）交联。结果表明，溶质吸附量和酸性磷酸酶在交联壳聚糖珠上的固定化能力受其交联程度的显著影响。壳聚糖与戊二醛的交联速率可用准二级方程描述，交联平衡可用弗伦德里希方程描述。这提供了一种控制壳聚糖珠交联程度的实验方法。最后，测量了固定化酶的活性和寿命以评估其应用潜力。
