Characterization of the lipA gene encoding the major lipase from Pseudomonas aeruginosa strain IGB83.

作者信息

Martínez A, Soberón-Chávez G

机构信息

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Cuernavaca, Morelos.

出版信息

Appl Microbiol Biotechnol. 2001 Sep;56(5-6):731-5. doi: 10.1007/s002530100724.

The lipases produced by Pseudomonas have a wide range of potential biotechnological applications. Pseudomonas aeruginosa IGB83 was isolated as a highly lipolytic strain which produced a thermotolerant and alkaline lipase. In the present work, we have characterized the P. aeruginosa IGB83 gene (lipA) encoding this enzyme. We describe the construction of a lipA mutant and report on the effect of two carbon sources on lipase expression.