Institute for Molecular Enzyme Technology, Research Centre Juelich, Heinrich-Heine-University Duesseldorf, Juelich, Germany.
FEMS Microbiol Lett. 2010 Aug 1;309(1):25-34. doi: 10.1111/j.1574-6968.2010.02017.x. Epub 2010 May 17.
Pseudomonas aeruginosa produces and secretes several lipolytic enzymes, among them the lipases LipA and LipC. LipA is encoded within the lipA/lipH operon, together with its cognate foldase LipH, which was also found to be required for the functional expression of LipC. At present, the physiological function of LipC is unknown. We have cloned a synthetic operon consisting of the lipC structural gene and the foldase gene lipH obtained from the lipA/lipH operon and have constructed, in parallel, a lipC-deficient P. aeruginosa mutant. Inactivation of the lipC gene significantly impaired type IV pilus-dependent twitching and swarming motility, but also the flagella-mediated swimming motility of P. aeruginosa. Moreover, for the lipC mutant, we observed a significant decrease in the amount of extracellular rhamnolipids. Also, the P. aeruginosa lipC mutant showed a significantly altered biofilm architecture. Proteome analysis revealed the accumulation of the response regulator protein PhoP in the lipC mutant.
铜绿假单胞菌产生并分泌几种脂解酶，其中包括脂肪酶 LipA 和 LipC。LipA 编码在 lipA/lipH 操纵子内，与其同源折叠酶 LipH 一起，也发现 LipH 对于 LipC 的功能表达是必需的。目前，LipC 的生理功能尚不清楚。我们已经克隆了一个由来自 lipA/lipH 操纵子的 lipC 结构基因和折叠酶基因 lipH 组成的合成操纵子，并平行构建了 lipC 缺陷型铜绿假单胞菌突变体。LipC 基因的失活显著削弱了 IV 型菌毛依赖的翻滚和群集运动，但也削弱了铜绿假单胞菌的鞭毛介导的游泳运动。此外，对于 lipC 突变体，我们观察到细胞外鼠李糖脂的量显著减少。此外，铜绿假单胞菌 lipC 突变体的生物膜结构发生了明显改变。蛋白质组分析显示响应调节蛋白 PhoP 在 lipC 突变体中积累。
