Danve C, Decaussin G, Busson P, Ooka T
Laboratoire de Virologie Moléculaire, IVMC, UMR5537, CNRS, Faculté de Médecine R.T.H. Laennec, Université Claude Bernard Lyon-1, 69372, Lyon Cedex 08, France.
Virology. 2001 Sep 30;288(2):223-35. doi: 10.1006/viro.2001.1072.
The Epstein-Barr virus (EBV) is associated with two major human epithelial malignancies, where it is likely to play a role in the malignant phenotype: undifferentiated nasopharyngeal carcinoma (100% of cases) and gastric carcinomas (about 10% of cases). We and others have obtained growth transformation of monkey kidney primary epithelial cells by transfection of viral DNA, especially with the BARF1 gene of EBV (Wei et al., 1997). We now report that the same type of primary epithelial cells can be growth-transformed using EBV particles derived from a nasopharyngeal carcinoma tumor line. Not only can these EBV-infected cells grow over 100 passages, escaping senescence, in contrast to their noninfected counterparts, but they can also survive and proliferate at very low cell density. Several subclones were characterized in terms of viral gene expression. All these clones gave a similar pattern, with detection of EBNA1 and BARF1 proteins but absence of LMP1. CD21, which is the main EBV receptor on B lymphocytes, was not expressed on parental monkey kidney epithelial cells nor on EBV-infected cell clones. This model of epithelial cell transformation will be useful for a better investigation of EBV functions critical for oncogenesis of epithelial cells.
爱泼斯坦-巴尔病毒（EBV）与两种主要的人类上皮恶性肿瘤有关，它可能在恶性表型中发挥作用：未分化鼻咽癌（100%的病例）和胃癌（约10%的病例）。我们和其他人通过转染病毒DNA，特别是EBV的BARF1基因，实现了猴肾原代上皮细胞的生长转化（Wei等人，1997年）。我们现在报告，使用源自鼻咽癌肿瘤系的EBV颗粒可以使同一类型的原代上皮细胞发生生长转化。与未感染的对应细胞相比，这些被EBV感染的细胞不仅可以传代超过100次而不衰老，而且还能在非常低的细胞密度下存活和增殖。对几个亚克隆进行了病毒基因表达方面的表征。所有这些克隆都呈现出相似的模式，检测到EBNA1和BARF1蛋白，但未检测到LMP1。CD21是B淋巴细胞上主要的EBV受体，在亲代猴肾上皮细胞和EBV感染的细胞克隆上均未表达。这种上皮细胞转化模型将有助于更好地研究对上皮细胞肿瘤发生至关重要的EBV功能。
