Martin D P, Willment J A, Billharz R, Velders R, Odhiambo B, Njuguna J, James D, Rybicki E P
Department of Moleculare Cell Biology, University of Cape Town, Cape Town, South Africa, 7701.
Virology. 2001 Sep 30;288(2):247-55. doi: 10.1006/viro.2001.1075.
Full genomic sequences were determined for 12 Maize streak virus (MSV) isolates obtained from Zea mays and wild grass species. These and 10 other publicly available full-length sequences were used to classify a total of 66 additional MSV isolates that had been characterized by PCR-restriction fragment length polymorphism and/or partial nucleotide sequence analysis. A description is given of the host and geographical distribution of the MSV strain and subtype groupings identified. The relationship between the genotypes of 21 fully sequenced virus isolates and their virulence in differentially MSV-resistant Z. mays genotypes was examined. Within the only MSV strain grouping that produced severe symptoms in maize, highly virulent and widely distributed genotypes were identified that are likely to pose the most serious threat to maize production in Africa. Evidence is presented that certain of the isolates investigated may be the products of either intra- or interspecific recombination.
测定了从玉米和野草物种中获得的12株玉米条纹病毒（MSV）分离株的全基因组序列。这些序列以及其他10个公开可用的全长序列被用于对另外66株MSV分离株进行分类，这些分离株此前已通过PCR-限制性片段长度多态性和/或部分核苷酸序列分析进行了鉴定。文中描述了所鉴定的MSV毒株和亚型分组的宿主及地理分布。研究了21株全序列病毒分离株的基因型与其在对MSV具有不同抗性的玉米基因型中的毒力之间的关系。在唯一一种在玉米中产生严重症状的MSV毒株分组中，鉴定出了高毒力且分布广泛的基因型，这些基因型可能对非洲的玉米生产构成最严重的威胁。有证据表明，所研究的某些分离株可能是种内或种间重组的产物。
