Kamiyama Tomoko, Kurokawa Masahiko, Shiraki Kimiyasu
Department of Virology, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan1.
J Gen Virol. 2001 Nov;82(Pt 11):2761-2765. doi: 10.1099/0022-1317-82-11-2761.
The nucleotide changes of the DNA polymerase gene and the susceptibility of acyclovir (ACV)-resistant varicella-zoster virus (VZV) mutants to anti-herpetic drugs were determined and compared to those of herpes simplex virus type 1 (HSV-1) mutants. The seven ACV-resistant VZV mutants were classified into three groups, N(779)S, G(805)C and V(855)M, according to the sequences of their DNA polymerase genes. The amino acid substitutions N(779)S and G(805)C were identical in position to the N(815)S and G(814)C mutations in the HSV-1 DNA polymerase mutants, respectively, and the V(855)M amino acid substitution was similar to the HSV-1 V(892)M mutation. All three groups of VZV mutants were susceptible to ACV, phosphonoacetic acid, vidarabine and aphidicolin, at levels similar to those seen with the respective HSV-1 mutants, except for subtle differences that were due possibly to the non-conserved regions in their sequences. Although both the HSV-1 and the VZV DNA polymerase genes show 53% sequence similarity, both viruses essentially show a similar biochemical behaviour.
测定了DNA聚合酶基因的核苷酸变化以及阿昔洛韦（ACV）耐药性水痘带状疱疹病毒（VZV）突变体对抗疱疹药物的敏感性，并与1型单纯疱疹病毒（HSV-1）突变体进行了比较。根据其DNA聚合酶基因序列，7株ACV耐药性VZV突变体被分为三组，即N(779)S、G(805)C和V(855)M。氨基酸替代N(779)S和G(805)C在位置上分别与HSV-1 DNA聚合酶突变体中的N(815)S和G(814)C突变相同，而V(855)M氨基酸替代与HSV-1的V(892)M突变相似。除了可能由于其序列中非保守区域导致的细微差异外，所有三组VZV突变体对ACV、膦甲酸、阿糖腺苷和阿非科林的敏感性与相应的HSV-1突变体相似。虽然HSV-1和VZV的DNA聚合酶基因序列相似性为53%，但两种病毒在本质上表现出相似的生化行为。
