金丝雀新型圆环病毒的核苷酸序列分析及其与圆环病毒科圆环病毒属其他成员的关系。

Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae.

作者信息

Phenix Kerry V, Weston Jonathan H, Ypelaar Ingrid, Lavazza Antonio, Smyth Joan A, Todd Daniel, Wilcox Graham E, Raidal Shane R

机构信息

Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia1.

Department of Veterinary Science, The Queen's University of Belfast, Stormont, Belfast BT4 3SD, Northern Ireland2.

出版信息

J Gen Virol. 2001 Nov;82(Pt 11):2805-2809. doi: 10.1099/0022-1317-82-11-2805.

DOI:10.1099/0022-1317-82-11-2805

PMID:11602793

Abstract

The circular, single-stranded DNA genome of a novel circovirus of canaries, tentatively named canary circovirus (CaCV), was cloned and sequenced. Sequence analysis indicated that the genome was 1952 nucleotides (nt) in size and had the potential to encode three viral proteins, including the putative capsid and replication-associated (Rep) proteins. The CaCV genome shared greatest sequence similarity (58.3% nt identity) with the newly characterized columbid circovirus (CoCV) and was more distantly related to the two porcine circovirus strains, PCV1 and PCV2, beak and feather disease virus (BFDV) and a recently isolated goose circovirus (GCV) isolate (46.8-50.9% nt identity). In common with other members of the Circovirus genus, several nt structures and amino acid motifs thought to be implicated in virus replication were identified on the putative viral strand. Phylogenetic analysis of both the capsid and Rep protein-coding regions provided further evidence that CaCV is more closely related to CoCV and BFDV and more distantly related to GCV, PCV1 and PCV2.

摘要

一种暂命名为金丝雀圆环病毒（CaCV）的新型金丝雀圆环病毒的环状单链DNA基因组被克隆并测序。序列分析表明，该基因组大小为1952个核苷酸（nt），有潜力编码三种病毒蛋白，包括假定的衣壳蛋白和复制相关（Rep）蛋白。CaCV基因组与新鉴定的鸽圆环病毒（CoCV）具有最大的序列相似性（核苷酸同一性为58.3%），与两种猪圆环病毒株PCV1和PCV2、喙羽病病毒（BFDV）以及最近分离的一种鹅圆环病毒（GCV）分离株的亲缘关系更远（核苷酸同一性为46.8 - 50.9%）。与圆环病毒属的其他成员一样，在假定的病毒链上鉴定出了几个被认为与病毒复制有关的核苷酸结构和氨基酸基序。对衣壳蛋白和Rep蛋白编码区的系统发育分析进一步证明，CaCV与CoCV和BFDV的亲缘关系更近，与GCV、PCV1和PCV2的亲缘关系更远。