Todd D, Weston J H, Soike D, Smyth J A
Department of Agriculture and Rural Development for Northern Ireland, Veterinary Sciences Division, Stormont, Belfast BT4 3SD, United Kingdom.
Virology. 2001 Aug 1;286(2):354-62. doi: 10.1006/viro.2001.0985.
The genomes of novel circoviruses from goose and pigeon, which were isolated using degenerate primer and inverse primer PCR methods, were cloned and sequenced. Comparative nucleotide (nt) sequence analyses showed that the goose circovirus (GCV) and pigeon circovirus (PiCV) possessed genomes which were 1821 and 2037 or 2036 nt, respectively, and which had features in common with the genomes of porcine circoviruses types 1 and 2 (PCV1, PCV2) and psittacine beak and feather disease virus (BFDV), such that they can now be assigned to the genus Circovirus of the family Circoviridae. Common features include the possession of (i) a potential stem-loop/nonanucleotide motif with which the initiation of rolling circle replication of the virus DNA is associated; (ii) two major ORFs, located on the virus (V1 ORF) and complementary (C1 ORF) strands, which encode the replication-associated protein (Rep) and capsid protein, respectively; (iii) high levels of amino acid identity (41.2--58.2%) shared with other circovirus Rep proteins; and (iv) direct/inverted repeat sequences within the putative intergenic region. On the basis of nt and amino acid sequence identities, GCV is substantially less closely related to BFDV than PiCV is to BFDV.
利用简并引物和反向引物PCR方法分离出的来自鹅和鸽的新型圆环病毒基因组，被克隆并测序。比较核苷酸（nt）序列分析表明，鹅圆环病毒（GCV）和鸽圆环病毒（PiCV）的基因组分别为1821和2037或2036 nt，并且具有与1型和2型猪圆环病毒（PCV1、PCV2）以及鹦鹉喙羽病病毒（BFDV）基因组相同的特征，因此它们现在可被归入圆环病毒科的圆环病毒属。共同特征包括：（i）拥有一个潜在的茎环/九核苷酸基序，病毒DNA的滚环复制起始与之相关；（ii）两个主要的开放阅读框，分别位于病毒链（V1 ORF）和互补链（C1 ORF）上，分别编码复制相关蛋白（Rep）和衣壳蛋白；（iii）与其他圆环病毒Rep蛋白具有较高的氨基酸同一性（41.2 - 58.2%）；（iv）假定基因间隔区内的正向/反向重复序列。基于核苷酸和氨基酸序列同一性，GCV与BFDV的亲缘关系远不如PiCV与BFDV密切。
