人妊娠母胎界面处二胺氧化酶及组胺受体H1和H2的mRNA定位
Localisation of mRNAs for diamine oxidase and histamine receptors H1 and H2, at the feto-maternal interface of human pregnancy.
作者信息
Brew O B, Sullivan M H
机构信息
Wolfson Institute of Health Science, Thames Valley University, London, UK.
出版信息
Inflamm Res. 2001 Sep;50(9):449-52. doi: 10.1007/PL00000269.
OBJECTIVE AND DESIGN
To localise mRNAs for the histamine receptors H1, H2 and H3, and for diamine oxidase, in the placenta and decidua of the human feto-maternal interface.
MATERIALS AND METHODS
Complementary DNA for each mRNA of interest was amplified by polymerase chain reaction. Sub-cloned sequences were used to prepare probes for in situ hybridisation, and these were employed to localise the expression of mRNAs for histamine receptors H1 and H2, and for diamine oxidase.
RESULTS
mRNA for histamine receptors H1 and H2, and for diamine oxidase could be detected at the feto-maternal interface of human pregnancy, and localised to both decidual and placental cells.
CONCLUSION
The co-expression of these receptors and DAO is consistent with a role for histamine at the feto-maternal interface of human pregnancy.
目的与设计
在人胎儿 - 母体界面的胎盘和蜕膜中定位组胺受体H1、H2和H3以及二胺氧化酶的mRNA。
材料与方法
通过聚合酶链反应扩增每种感兴趣mRNA的互补DNA。亚克隆序列用于制备原位杂交探针，并用于定位组胺受体H1和H2以及二胺氧化酶mRNA的表达。
结果
在人类妊娠的胎儿 - 母体界面可检测到组胺受体H1和H2以及二胺氧化酶的mRNA，并定位于蜕膜和胎盘细胞。
结论
这些受体与二胺氧化酶的共表达与人妊娠胎儿 - 母体界面处组胺的作用一致。
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