Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Gynecol Obstet Invest. 2010;69(1):67-72. doi: 10.1159/000257663. Epub 2009 Nov 12.
BACKGROUND/AIMS: Previous studies have stated that maternal allergic diseases are associated with increased risk of preterm labor/delivery, but the underlying mechanisms remain unclear. This study tested the hypothesis that histamine induces interleukin (IL)-6 production in amnion cells.
Using cultured human amnion cells, we examined expression of histamine receptors and effects of histamine on IL-6 production.
Reverse transcription-polymerase chain reaction and Western blotting revealed expression of histamine H1 receptor (H1R) and H2 receptor (H2R) in human amnion. Histamine stimulation significantly increased concentrations of IL-6 in conditioned medium, as did tumor necrosis factor-alpha and IL-1beta in positive controls. In addition, the H1R antagonist olopatadine significantly blocked histamine-induced production of IL-6, whereas the H2R antagonist ranitidine did not.
Histamine appears to induce IL-6 production through H1R in human amnion cells.
背景/目的：先前的研究表明，母体过敏疾病与早产/分娩的风险增加有关，但潜在的机制尚不清楚。本研究检验了这样一个假设，即组胺诱导羊膜细胞产生白细胞介素（IL）-6。
我们使用培养的人羊膜细胞，检测了组胺受体的表达以及组胺对 IL-6 产生的影响。
逆转录-聚合酶链反应和 Western blot 显示人羊膜中存在组胺 H1 受体（H1R）和 H2 受体（H2R）的表达。组胺刺激显著增加了条件培养基中 IL-6 的浓度，阳性对照物肿瘤坏死因子-α和 IL-1β也是如此。此外，H1R 拮抗剂奥洛他定显著阻断了组胺诱导的 IL-6 产生，而 H2R 拮抗剂雷尼替丁则没有。
组胺似乎通过人羊膜细胞中的 H1R 诱导 IL-6 的产生。
