Last J A, Waggoner T A, Sasaki D Y
Biomolecular Materials and Interfaces Department, Sandia National Laboratories, Albuquerque, New Mexico 87185, USA.
Biophys J. 2001 Nov;81(5):2737-42. doi: 10.1016/S0006-3495(01)75916-4.
Nanoscale structural reorganization of a lipid bilayer membrane induced by a chemical recognition event has been imaged using in situ atomic force microscopy (AFM). Supported lipid bilayers, composed of distearylphosphatidylcholine (DSPC) and a synthetic lipid functionalized with a Cu(2+) receptor, phase-separate into nanoscale domains that are distinguishable by the 9 A height difference between the two molecules. Upon binding of Cu(2+) the electrostatic nature of the receptor changes, causing a dispersion of the receptor molecules and subsequent shrinking of the structural features defined by the receptors in the membrane. Complete reversibility of the process was demonstrated through the removal of metal ions with EDTA.
利用原位原子力显微镜（AFM）对由化学识别事件诱导的脂质双分子层膜的纳米级结构重组进行了成像。由二硬脂酰磷脂酰胆碱（DSPC）和用Cu(2+)受体功能化的合成脂质组成的支撑脂质双分子层相分离成纳米级区域，这两个分子之间9埃的高度差可区分这些区域。Cu(2+)结合后，受体的静电性质发生变化，导致受体分子分散，随后膜中由受体定义的结构特征收缩。通过用乙二胺四乙酸（EDTA）去除金属离子，证明了该过程的完全可逆性。
