Lee H I, León J, Raskin I
AgBiotech Center, Cook College, Rutgers University, New Brunswick, NJ 08903-0231, USA.
Proc Natl Acad Sci U S A. 1995 May 9;92(10):4076-9. doi: 10.1073/pnas.92.10.4076.
Pathways of salicylic acid (SA) biosynthesis and metabolism in tobacco have been recently identified. SA, an endogenous regulator of disease resistance, is a product of phenylpropanoid metabolism formed via decarboxylation of trans-cinnamic acid to benzoic acid and its subsequent 2-hydroxylation to SA. In tobacco mosaic virus-inoculated tobacco leaves, newly synthesized SA is rapidly metabolized to SA O-beta-D-glucoside and methyl salicylate. Two key enzymes involved in SA biosynthesis and metabolism: benzoic acid 2-hydroxylase, which converts benzoic acid to SA, and UDPglucose:SA glucosyltransferase (EC 2.4.1.35), which catalyzes conversion of SA to SA glucoside have been partially purified and characterized. Progress in enzymology and molecular biology of SA biosynthesis and metabolism will provide a better understanding of signal transduction pathway involved in plant disease resistance.
烟草中水杨酸(SA)生物合成与代谢途径最近已被确定。SA是一种抗病性的内源性调节因子,是苯丙烷类代谢的产物,由反式肉桂酸脱羧形成苯甲酸,随后苯甲酸经2-羟基化生成SA。在接种烟草花叶病毒的烟草叶片中,新合成的SA迅速代谢为SA O-β-D-葡萄糖苷和水杨酸甲酯。参与SA生物合成与代谢的两种关键酶:将苯甲酸转化为SA的苯甲酸2-羟化酶,以及催化SA转化为SA葡萄糖苷的UDP葡萄糖:SA葡萄糖基转移酶(EC 2.4.1.35)已被部分纯化并进行了特性鉴定。SA生物合成与代谢的酶学和分子生物学进展将有助于更好地理解植物抗病性所涉及的信号转导途径。