Integrating Transcriptomics and Metabolomics to Comprehensively Analyze Phytohormone Regulatory Mechanisms in Pall. Under UV-B Radiation.

In order to fully elucidate the roles and systems of phytohormones in UV-B radiation (UV-B) leaves of the Pall. (), we conducted a comprehensive analysis of how protects itself against UV-B using transcriptomic and metabolomic data. Transcript and metabolite profiles were generated by a combination of deep sequencing and LC-MS/MS (liquid chromatography-tandem mass spectrometry), respectively. Combined with physiological and biochemical assays, we studied compound accumulation, biosynthesis and expression of signaling genes of seven hormones and the effects of hormones on plant photosynthesis. The findings indicate that during leaf defense against UV-B, photosynthesis declined, the photosynthetic system was impaired and the concentration of salicylic acid (SA) hormones increased, whereas the contents of cytokinin (CK), abscisic acid (ABA), ethylene, auxin, jasmonic acid (JA) and gibberellins (GAs) continued to decrease. Finally, correlation tests between hormone content and genes were analyzed, and genes closely related to leaf resistance to UV-B were identified in seven pathways. These results will expand our understanding of the hormonal regulatory mechanisms of plant resistance to UV-B and at the same time lay the foundation for plant resistance to adversity stress.