通过补骨脂素交联的光化学逆转分析RNA二级结构。
Analysis of RNA secondary structure by photochemical reversal of psoralen crosslinks.
作者信息
Rabin D, Crothers D M
出版信息
Nucleic Acids Res. 1979 Oct 10;7(3):689-703. doi: 10.1093/nar/7.3.689.
Abstract
Aminomethyltrioxsalen (AMT), a psoralen, is known to cause interstrand crosslinks in double stranded nucleic acids. We have demonstrated the photochemical reversal of this reaction, and have used this result to develop a method for identification of specific sequences which are adjacent because of RNA secondary structure formation. E. coli 5S rRNA is used as a model system. We isolated and characterized a product that is derived from the stem region of 5S RNA.
摘要
氨基甲基三氧杂蒽酮（AMT）是一种补骨脂素，已知它会在双链核酸中引起链间交联。我们已经证明了该反应的光化学逆转，并利用这一结果开发了一种鉴定因RNA二级结构形成而相邻的特定序列的方法。大肠杆菌5S rRNA被用作模型系统。我们分离并鉴定了一种源自5S RNA茎区的产物。
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