大肠杆菌16S核糖体RNA中补骨脂素交联位点的鉴定。
Identification of a site of psoralen crosslinking in E. coli 16S ribosomal RNA.
作者信息
Turner S, Thompson J F, Hearst J E, Noller H F
出版信息
Nucleic Acids Res. 1982 May 11;10(9):2839-49. doi: 10.1093/nar/10.9.2839.
Abstract
We have developed a 2-dimensional gel method for identification of RNA sequences crosslinked by the intercalative drug 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). This method is being used to localize such sites in E. coli ribosomal RNA. We report here the identification of a site for HMT crosslinking within positions 434 and 497 of 16S rRNA. We suggest a likely site for HMT intercalation, in which residues U548 and U473 become crosslinked via the drug.
摘要
我们开发了一种二维凝胶方法，用于鉴定由嵌入药物4'-羟甲基-4,5',8-三甲基补骨脂素（HMT）交联的RNA序列。该方法正用于在大肠杆菌核糖体RNA中定位此类位点。我们在此报告在16S rRNA的434位和497位之间鉴定出一个HMT交联位点。我们提出了一个HMT嵌入的可能位点，其中U548和U473残基通过该药物发生交联。
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