Leppard G G
Science. 1975 Sep 26;189(4208):1094-5. doi: 10.1126/science.1162359.
Freeze-etching of never-dried pellicles or of incubated suspensions of both Acetobacter xylinum and Acetobacter acetigenum show a nascent form of the cellulose microfibril which has a core surrounded by an amorphous sheath. Drying of the pellicle or suspension reduces the diameter of the sheath and changes the form of the microfibril to the one usually seen. This nascent form of the cellulose microfibril is consistent with previous postulations of an intermediate polymer or polymers in the biosynthesis of cellulose.
对未干燥的菌膜或木醋杆菌和产乙酸醋杆菌的培养悬浮液进行冷冻蚀刻，结果显示出纤维素微纤丝的一种新生形式，其具有被无定形鞘包围的核心。菌膜或悬浮液的干燥会减小鞘的直径，并将微纤丝的形态改变为通常所见的形态。纤维素微纤丝的这种新生形式与先前关于纤维素生物合成中一种或多种中间聚合物的推测一致。
