Haigler C H, White A R, Brown R M, Cooper K M
J Cell Biol. 1982 Jul;94(1):64-9. doi: 10.1083/jcb.94.1.64.
In vivo cellulose ribbon assembly by the Gram-negative bacterium Acetobacter xylinum can be altered by incubation in carboxymethylcellulose (CMC), a negatively charged water-soluble cellulose derivative, and also by incubation in a variety of neutral, water-soluble cellulose derivatives. In the presence of all of these substituted celluloses, normal fasciation of microfibril bundles to form the typical twisting ribbon is prevented. Alteration of ribbon assembly is most extensive in the presence of CMC, which often induces synthesis of separate, intertwining bundles of microfibrils. Freeze-etch preparations of the bacterial outer membrane suggest that particles that are thought to be associated with cellulose synthesis or extrusion may be specifically organized to mediate synthesis of microfibril bundles. These data support the previous hypothesis that the cellulose ribbon of A. xylinum is formed by a hierarchical, cell-directed, self-assembly process. The relationship of these results to the regulation of cellulose microfibril size and wall extensibility in plant cell walls is discussed.
革兰氏阴性细菌木醋杆菌在体内进行的纤维素带组装，可通过在羧甲基纤维素（CMC，一种带负电荷的水溶性纤维素衍生物）中孵育，以及在多种中性水溶性纤维素衍生物中孵育而发生改变。在所有这些取代纤维素存在的情况下，微纤丝束正常束化形成典型的扭曲带的过程会受到阻碍。在CMC存在时，带组装的改变最为广泛，CMC常常诱导单独的、相互缠绕的微纤丝束的合成。细菌外膜的冷冻蚀刻制剂表明，那些被认为与纤维素合成或挤出相关的颗粒可能经过特殊组织，以介导微纤丝束的合成。这些数据支持了先前的假设，即木醋杆菌的纤维素带是通过一个分级的、细胞导向的自组装过程形成的。本文还讨论了这些结果与植物细胞壁中纤维素微纤丝大小调节和壁伸展性的关系。
