Chimishkian K L, Svet-Moldavskiĭ G Ia
Vopr Virusol. 1975 May-Jun(3):273-8.
Injection of living vaccinia virus to the mesenteric vein of rabbits leads to its accumulation in liver in high concentration. In the other series of experiments vaccinia virus inactivated with gamma-radiation was inoculated into the mesenteric vein. Later on the animals were sacrificed at various dates. Homogenates were prepared from their liver. Comparative study of these homogenates made it possible to reveal increased immunogenic activity of homogenate obtained 24 hours after intravenous inoculation of inactivated vaccinia virus into animals. Immunogenic activity of this homogenate was more clearly manifested than immunogenic activity of the antigen itself--inactivated vaccinia vorus. Neither original inactivated vaccinia virus nor inactivated virus absorbed by Kupffer cells induced antibody formation. It is supposed that vaccines may be prepared from various viruses or tumor cells treated with macrophages with subsequent chemical extraction of the most immunogenic fraction.
将活痘苗病毒注入兔的肠系膜静脉会使其在肝脏中高浓度蓄积。在另一系列实验中，将经γ射线灭活的痘苗病毒接种到肠系膜静脉中。随后在不同时间处死动物。从它们的肝脏制备匀浆。对这些匀浆进行比较研究后发现，在给动物静脉接种灭活痘苗病毒24小时后获得的匀浆免疫原性活性增强。这种匀浆的免疫原性活性比抗原本身——灭活痘苗病毒的免疫原性活性表现得更明显。无论是原始的灭活痘苗病毒还是被库普弗细胞吸收的灭活病毒都不能诱导抗体形成。据推测，可以用巨噬细胞处理各种病毒或肿瘤细胞，随后化学提取免疫原性最强的部分来制备疫苗。
