Zaror L, Robles A M, Negroni R
Rev Asoc Argent Microbiol. 1978 May-Aug;10(2):61-4.
Different immunodiffusion techniques with and without the addition of polyetilenglycol 6000 (PEG), were studied to determine its effect on the sensitivity of these reactions. One hundred thirteen sera from patients who suffered or had suffered deep mycoses (paracoccidioidomycosis: 49, histoplasmosis: 25, aspergillosis: 25, candidiasis: 8 and coccidioidomycosis: 6) were examined by the quantitative Ouchterlony's immunodiffusion procedure. Regular medium and media with 2% and 4% PEG were used. Eighty two out of the one hundred thirteen sera were positive for the regular medium and 91 for the medium containing 2% of PEG; furthermore, an increase of 1 or 2 two fold dilutions in the titers was observed in 40% of the sera, for the later media. Twenty one sera from aspergillosis cases were examined by agarose gel immunoelectrophoresis, 80% had more precipitin bands in the medium with 2% of PEG. Thirty four serum samples of patients suffering aspergillosis, paracoccidioidomycosis and histoplasmosis were studied using the agarose electroosmophoresis with the secondary immunodiffusion test. An increase in the number of the anodic bands were observed in 55% while 64% presented more catodic bands, when the PEG medium was used. This results would indicate that the addition of 2% PEG 6000 to the regular medium improves the sensitivity of the immunodiffusion tests for mycoses.
研究了添加和不添加聚乙二醇6000（PEG）的不同免疫扩散技术，以确定其对这些反应敏感性的影响。采用定量奥克特洛尼免疫扩散法检测了113例患有或曾患有深部真菌病（副球孢子菌病：49例，组织胞浆菌病：25例，曲霉病：25例，念珠菌病：8例，球孢子菌病：6例）患者的血清。使用常规培养基以及添加2%和4% PEG的培养基。在113份血清中，82份在常规培养基中呈阳性，91份在含2% PEG的培养基中呈阳性；此外，对于后一种培养基，40%的血清滴度增加了1或2倍稀释度。对21份曲霉病病例的血清进行琼脂糖凝胶免疫电泳检测，80%在含2% PEG的培养基中有更多沉淀带。使用琼脂糖电渗电泳结合二次免疫扩散试验，对34例患有曲霉病、副球孢子菌病和组织胞浆菌病患者的血清样本进行了研究。当使用PEG培养基时，55%的样本阳极带数量增加，64%的样本阴极带数量更多。这些结果表明，在常规培养基中添加2%的PEG 6000可提高真菌病免疫扩散试验的敏感性。
