Shimizu S, Kataoka M, Honda K, Sakamoto K
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
J Biotechnol. 2001 Dec 28;92(2):187-94. doi: 10.1016/s0168-1656(01)00359-5.
Microbial lactonohydrolases (lactone-ring-cleaving enzymes) with unique characteristics were found. The Fusarium oxysporum enzyme catalyzes the reversible and stereospecific hydrolysis of aldonate lactones and D-pantolactone (D-PL), and is useful for the optical resolution of racemic PL. The Agrobacterium tumefaciens enzyme hydrolyzes several aromatic lactones, and catalyzes the stereospecific hydrolysis of PL like the Fusarium enzyme, but its selectivity is opposite. The Acinetobacter calcoaceticus enzyme catalyzing the specific hydrolysis of dihydrocoumarin belongs to serine-enzyme family, and is useful for enantioselective hydrolysis of methyl DL-beta-acetylthioisobutyrate and regioselective hydrolysis of methyl cetraxate. This enzyme also catalyzes the bromination of monochlorodimedon when incubated with H(2)O(2) and dihydrocoumarin.
发现了具有独特特性的微生物内酯水解酶（内酯环裂解酶）。尖孢镰刀菌酶催化醛糖酸内酯和D -泛内酯（D - PL）的可逆和立体特异性水解，可用于外消旋PL的光学拆分。根癌土壤杆菌酶水解几种芳香族内酯，并像镰刀菌酶一样催化PL的立体特异性水解，但其选择性相反。醋酸钙不动杆菌酶催化二氢香豆素的特异性水解，属于丝氨酸酶家族，可用于对映选择性水解DL -β-乙酰硫代异丁酸甲酯和区域选择性水解塞曲司特甲酯。当与H₂O₂和二氢香豆素一起孵育时，该酶还催化一氯二甲基酮的溴化反应。
