小鼠6号染色体上蔗糖八乙酸酯味觉厌恶（Soa）位点的高分辨率遗传图谱。
High-resolution genetic mapping of the sucrose octaacetate taste aversion (Soa) locus on mouse Chromosome 6.
作者信息
Bachmanov A A, Li X, Li S, Neira M, Beauchamp G K, Azen E A
机构信息
Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
出版信息
Mamm Genome. 2001 Sep;12(9):695-9. doi: 10.1007/s00335-001-2061-7.
An acetylated sugar, sucrose octaacetate (SOA), tastes bitter to humans and has an aversive taste to at least some mice and other animals. In mice, taste aversion to SOA depends on allelic variation of a single locus, Soa. Three Soa alleles determine 'taster' (Soa(a)), 'nontaster' (Soa(b)), and 'demitaster' (Soa(c)) phenotypes of taste sensitivity to SOA. Although Soa has been mapped to distal Chromosome (Chr) 6, the limits of the Soa region have not been defined. In this study, mice from congenic strains SW.B6-Soa(b), B6.SW-Soa(a), and C3.SW-Soa(a/c) and from an outbred CFW strain were genotyped with polymorphic markers on Chr 6. In the congenic strains, the limits of introgressed donor fragments were determined. In the outbred mice, linkage disequilibrium and haplotype analyses were conducted. Positions of the markers were further resolved by using radiation hybrid mapping. The results show that the Soa locus is contained in an approximately 1-cM (3.3-4.9 Mb) region including the Prp locus.
一种乙酰化糖，八乙酸蔗糖酯（SOA），对人类来说味道苦涩，并且对至少一些小鼠和其他动物具有厌恶味道。在小鼠中，对SOA的味觉厌恶取决于一个单一基因座Soa的等位基因变异。三个Soa等位基因决定了对SOA味觉敏感性的“品尝者”（Soa(a)）、“非品尝者”（Soa(b)）和“半品尝者”（Soa(c)）表型。尽管Soa已被定位到6号染色体（Chr）远端，但Soa区域的界限尚未确定。在本研究中，对同源近交系SW.B6-Soa(b)、B6.SW-Soa(a)和C3.SW-Soa(a/c)以及远交CFW品系的小鼠进行了6号染色体上多态性标记的基因分型。在同源近交系中，确定了导入供体片段的界限。在远交小鼠中，进行了连锁不平衡和单倍型分析。通过使用辐射杂种图谱进一步解析了标记的位置。结果表明，Soa基因座包含在一个大约1厘摩（3.3 - 4.9兆碱基）的区域内，该区域包括Prp基因座。
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