Mouse Genomics Resource Laboratory, National Institute of Genetics, Mishima, Japan.
Behav Genet. 2011 Sep;41(5):716-23. doi: 10.1007/s10519-011-9464-3. Epub 2011 Apr 3.
Based on crosses among inbred strains derived principally from M. m. domesticus, sucrose octaacetate (SOA) aversion in laboratory mice has been thought for many years to be controlled by a single genetic locus (Soa) located on distal chromosome (Chr) 6. To expand knowledge of the genetic basis underlying SOA aversion, we have studied the M. m. molossinus derived strain (MSM) and MSM consomic strains on a M. m. domesticus (C57BL/6J: B6) host background. Using two-bottle preference procedures, MSM mice avoided 0.1 mM and 1 mM SOA while B6 mice were indifferent to 0.1 mM and exhibited slight aversion to 1 mM SOA. Preference tests of 16 available consomic strains implicated Chr 2, 4 and 15 in SOA aversion in addition to the prominent effect of the known Soa locus on Chr 6 (implicated by study of two congenic strains). The originally defined Soa locus is presumably associated with one or more members of the cluster of Tas2r genes on distal Chr 6 that code for bitter taste receptors. Our results point to the possible role of established Tas2r genes on Chr 2 and 15, as well as to genes not coding for bitter receptors (Chr 4), in SOA aversion. SOA aversion emerges from this consomic screen as being definitively under polygenic control. The genetic diversity captured by the MSM model system is shown to be a valuable tool to complement the limited genetic variation in the commonly used stocks derived from M m. domesticus.
基于主要来源于 M. m. domesticus 的近交系杂交，实验室小鼠对蔗糖八乙酸酯 (SOA) 的厌恶多年来被认为受位于远端染色体 (Chr) 6 上的单一遗传位点 (Soa) 控制。为了扩展对 SOA 厌恶遗传基础的了解，我们研究了源自 M. m. molossinus 的品系 (MSM) 和在 M. m. domesticus (C57BL/6J: B6) 宿主背景下的 MSM 同源染色体品系。使用双瓶偏好程序，MSM 小鼠回避 0.1 mM 和 1 mM SOA，而 B6 小鼠对 0.1 mM 无差异，对 1 mM SOA 表现出轻微厌恶。16 种可用的同源染色体品系的偏好测试表明，除了已知的 Soa 基因座对 Chr 6 的显著影响（通过对两种同基因品系的研究暗示）外，Chr 2、4 和 15 也参与了 SOA 厌恶。最初定义的 Soa 基因座可能与位于远端 Chr 6 上的 Tas2r 基因簇的一个或多个成员相关，这些基因编码苦味受体。我们的结果表明，在 Chr 2 和 15 上已建立的 Tas2r 基因以及不编码苦味受体的基因 (Chr 4) 可能在 SOA 厌恶中起作用。从这个同源染色体筛选中，SOA 厌恶被明确地认为是多基因控制的。MSM 模型系统所捕获的遗传多样性被证明是一种很有价值的工具，可以补充源自 M. m. domesticus 的常用品系中有限的遗传变异。
