El-Ackad T M, Brody M J
Blood Vessels. 1975;12(3):181-91. doi: 10.1159/000158049.
Histamine has been found biochemically in the mammalian vascular wall. This study was undertaken in an effort to identify the presence of specific histamine-containing cells inwalls of large blood vessels such as femoral artery and vein, brachial artery and vein, mesenteric artery and vein, renal artery and vein, and aorta. Segments of vessels from rats, cats, dogs, sheep, cows, humans and chickens were examined. Sections were prepared from fresh tissue by vibratome, from frozen tissue by cryostat and from freeze-dried and fixed tissues. Mast cells were visualized by staining with acidic toluidine blue and by reaction with orthophthaladehyde to develop histamine fluorescence. Although mast cells were easily identified in preparations such as canine liver, ear and scrotal skin and rat tongue, no such cells were found in any species (except the cow) in any part of the vascular wall. The results indicate that in most species histamine is stored in the vascular wall in a non-mast cell pool which may well be in the smooth muscle cells themselves.
已通过生化方法在哺乳动物血管壁中发现组胺。进行本研究旨在确定在诸如股动脉和静脉、肱动脉和静脉、肠系膜动脉和静脉、肾动脉和静脉以及主动脉等大血管壁中是否存在特定的含组胺细胞。对来自大鼠、猫、狗、绵羊、牛、人类和鸡的血管段进行了检查。通过振动切片机从新鲜组织制备切片，通过低温恒温器从冷冻组织制备切片，以及从冻干和固定组织制备切片。通过用酸性甲苯胺蓝染色以及与邻苯二醛反应以产生组胺荧光来观察肥大细胞。尽管在诸如犬肝、耳和阴囊皮肤以及大鼠舌等标本中很容易识别出肥大细胞，但在任何物种（牛除外）的血管壁任何部位均未发现此类细胞。结果表明，在大多数物种中，组胺存储在血管壁的非肥大细胞池中，该池很可能存在于平滑肌细胞自身中。
