果蝇物种与一个相关属之间的DNA同源性。

DNA homologies among Drosophila species and a related genus.

作者信息

Chakrabartty P K

出版信息

J Hered. 1975 Jul-Aug;66(4):213-7. doi: 10.1093/oxfordjournals.jhered.a108615.

DOI:10.1093/oxfordjournals.jhered.a108615

Abstract

Deoxyribonucleic acids of members of the family Drosophilidae; e.g., D. melanogaster, D. robusta, D. pellewae, D, immigrans, D. mcclintockae, D. calloptera, C. procnemis and from a tissue culture cells of D. melanogaster have been compared with respect to base composition, heterogenecity, and nucleotide sequence homology. Considerable heterogeneity exists in DNA's from 3rd instar larvae and tissue culture cells. The DNA base composition of adult species ranges from 33-42 moles percent GC; in addition a polydAT component is apparent in larval DNA's. There are about 21 and 29 percent intragenomic homology in DNA's of D. melanogaster and D. immigrans, respectively. Relatively large differences were revealed in the nucleotide sequences of several species by DNA hybridization and thermal stability studies.

摘要

果蝇科成员的脱氧核糖核酸；例如，黑腹果蝇、粗壮果蝇、佩氏果蝇、迁徙果蝇、麦克林托克果蝇、美翅果蝇、原蚊果蝇，以及黑腹果蝇的组织培养细胞，已在碱基组成、异质性和核苷酸序列同源性方面进行了比较。三龄幼虫和组织培养细胞的DNA存在相当大的异质性。成年物种的DNA碱基组成范围为33 - 42摩尔百分比的GC；此外，幼虫DNA中明显存在多聚AT成分。黑腹果蝇和迁徙果蝇的DNA分别有约21%和29%的基因组内同源性。通过DNA杂交和热稳定性研究发现，几个物种的核苷酸序列存在相对较大的差异。