Fehér Z, Schablik M, Szabó G
Acta Biol Acad Sci Hung. 1979;30(4):387-92.
Deoxyribonucleic acids of Neurospora crassa strains involved in genetic transformation experiments were studied by means of DNA-DNA hybridization. No significant difference was detected in the extent of hybridization reassociating 32P-DNA of an inositol-requiring recipient strain with an excess amount of unlabelled homologous DNA and that of the transformed, spontaneous revertant and wild-type strains. Studies on the thermal stability of hybrids revealed 1.2-1.7% heterology between the recipient and transformant DNA's. The spontaneous revertant and wild-type strains proved to be homologous with the recipient strain. We suppose that the heterology we measured is the result of the alteration of the nucleotide sequences caused by the multilocal integration of transforming DNA into the recipient genome.
利用DNA-DNA杂交技术研究了参与遗传转化实验的粗糙脉孢菌菌株的脱氧核糖核酸。将需要肌醇的受体菌株的32P-DNA与过量未标记的同源DNA杂交复性，以及将转化菌株、自发回复突变菌株和野生型菌株的32P-DNA与之杂交复性，在杂交程度上未检测到显著差异。对杂交体热稳定性的研究表明，受体DNA与转化体DNA之间存在1.2%-1.7%的异源性。自发回复突变菌株和野生型菌株与受体菌株同源。我们推测，我们所测量到的异源性是由转化DNA多位点整合到受体基因组中导致核苷酸序列改变的结果。
