Yamamoto G K, Allansmith M R
Am J Ophthalmol. 1979 Oct;88(4):758-63. doi: 10.1016/0002-9394(79)90679-2.
Tears from ten normal persons were used in hemolytic assays to test for activity of total hemolytic complement (tested in all ten subjects), activities of each of the nine complement components (tested in two subjects), and activity of the alternate pathway (tested in two subjects). A modified radial-immunodiffusion method was used to confirm the presence of C3 and C4 complement proteins in the tear samples from all ten subjects. Factor B protein was detected in tears from two of four normal subjects tested. In dilutions up to 1:4, hemolytic activity was observed in tears from five of the ten subjects. Samples from the remaining five subjects had CH50 activity at dilutions up to 1:2. In control tests, human serum had CH50 activity at 1:32. No lysis occurred in Veronal-buffered saline or in serum and tears heated to 56 degrees C for 30 minutes. Complement proteins C3 through C9 displayed hemolytic activity in tears from each of two subjects tested for complement components. The classic and alternate complement pathways in tears can be included among the defense mechanisms of the ocular surfaces.
从十名正常人获取的眼泪用于溶血试验，以检测总溶血补体的活性（对所有十名受试者进行检测）、九种补体成分中每种成分的活性（对两名受试者进行检测）以及替代途径的活性（对两名受试者进行检测）。采用改良的放射免疫扩散法来确认所有十名受试者眼泪样本中C3和C4补体蛋白的存在。在四名接受检测的正常受试者中，有两名的眼泪中检测到了B因子蛋白。在高达1:4的稀释度下，十名受试者中有五名的眼泪观察到溶血活性。其余五名受试者的样本在高达1:2的稀释度下具有CH50活性。在对照试验中，人血清在1:32时具有CH50活性。在巴比妥缓冲盐水或加热至56摄氏度30分钟的血清和眼泪中未发生裂解。在对补体成分进行检测的两名受试者中，补体蛋白C3至C9在其眼泪中均表现出溶血活性。眼泪中的经典和替代补体途径可纳入眼表的防御机制之中。
