一种通过梯度离心从外周血中分离和浓缩微丝蚴的技术。
A technique for isolating and concentrating microfilariae from peripheral blood by gradient centrifugation.
作者信息
Jones T C, Mott K, Pedrosa L C
出版信息
Trans R Soc Trop Med Hyg. 1975;69(2):243-6. doi: 10.1016/0035-9203(75)90162-5.
PMID:1166495
Abstract
A method for isolating microfilariae from blood cells by Ficoll-Hypaque centrifugation, followed by concentration by conventional centrifugation, is described. A 30 fold concentration and quantitative recovery of parasites was achieved. This technique should be of value for documenting low levels of parasitaemia, and for obtaining relatively pure suspensions of microfilariae for metabolic studies and antigen analysis.
摘要
本文描述了一种通过Ficoll-Hypaque离心法从血细胞中分离微丝蚴，随后通过常规离心法进行浓缩的方法。该方法实现了寄生虫30倍的浓缩和定量回收。这项技术对于记录低水平的寄生虫血症，以及获取相对纯净的微丝蚴悬浮液用于代谢研究和抗原分析具有重要价值。
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