Long G W, Rickman L S, Cross J H
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.
J Parasitol. 1990 Apr;76(2):278-81.
A microhematocrit tube technique for diagnosis of human filariasis has been previously described. A system incorporating heparin, EDTA, and acridine orange into a microhematocrit tube (Quantitative Blood Count, QBC) has been commercially developed for the quantitation of blood counts and has been used for the diagnosis of malaria. We evaluated this test for its usefulness in the diagnosis of filariasis. Upon centrifugation, the parasites were concentrated in the area of the buffy coat and could be observed through the wall of the tube. The parasites were concentrated further by a plastic float that expands the buffy coat and confines the parasites to the periphery of the tube. Acridine orange stains the DNA of the parasite, and morphologic characteristics can be examined by fluorescence microscopy. The terminal and subterminal nuclei and long cephalic space of Brugia malayi, as well as the short cephalic space and caudal nuclei of Wuchereria bancrofti, were easily recognized and differentiated from each other. Microfilariae were detected in samples diluted to a level of approximately 50/ml.
先前已描述了一种用于诊断人类丝虫病的微量血细胞比容管技术。一种将肝素、乙二胺四乙酸（EDTA）和吖啶橙加入微量血细胞比容管的系统（定量血细胞计数，QBC）已商业化开发用于血细胞计数定量，并已用于疟疾诊断。我们评估了该检测方法在丝虫病诊断中的实用性。离心后，寄生虫集中在血沉棕黄层区域，可通过管壁观察到。通过一个塑料浮子进一步浓缩寄生虫，该浮子可扩大血沉棕黄层并将寄生虫限制在管的周边。吖啶橙可对寄生虫的DNA进行染色，其形态特征可通过荧光显微镜检查。马来布鲁线虫的末端和亚末端细胞核以及长头间隙，以及班氏吴策线虫的短头间隙和尾核很容易识别且相互区分。在稀释至约50/毫升水平的样本中检测到了微丝蚴。
