Fokin S I, Osipov D V
Tsitologiia. 1975 Sep;17(9):1073-80.
The work is the first attempt to study nuclear dualism of ciliates with ultraviolet microbeam (UV-beam), which was not applied earlier for these purposes. The special advantage of the UV-beam is that it allow to inactivate selectively of the particular elements of nuclear apparatus of living ciliates is to observe consequences of operation on distant descendants of irradiated cell. On Paramecium caudatum the ways of UV-beam have been devised specifically. The original rotocompression chamber was employed for immobilization of cell in space of irradiation. The effective UV-doses were selected, the relative resistance of cytoplasm, macro-, micronucleus (Mi), and symbiotic bacteria of Mi -- omega particles -- was detected. The "pure" and infected cells were irradiated, in some different variants. The effect of UV-beam depends on dose and consists in a reduction the effectiveness of cell cloning, the loss of omega-particles from Mi, the appearance of the cells with 2--3 Mi, and, possibly, of amicronucleate cells. The injuries due to UV-beam are restored by photoreactivation of visible light.
这项工作是首次尝试用紫外微束（UV 束）研究纤毛虫的核二元论，此前尚未将其用于这些目的。UV 束的特殊优势在于它能够选择性地使活纤毛虫核装置的特定元件失活，从而观察对受辐照细胞的远代后代操作的后果。针对尾草履虫专门设计了 UV 束的照射方式。最初的旋转压缩室用于在照射空间中固定细胞。选择了有效的 UV 剂量，检测了细胞质、大核、小核（Mi）以及 Mi 的共生细菌——ω 颗粒的相对抗性。对“纯”细胞和感染细胞进行了不同变体的照射。UV 束的作用取决于剂量，表现为细胞克隆效率降低、ω 颗粒从小核丢失、出现具有 2 - 3 个小核的细胞，以及可能出现无小核细胞。UV 束造成的损伤可通过可见光的光复活作用恢复。
