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[生态长波波段中紫外线损伤的细胞和噬菌体的光复活作用]

[Photoreactivation of cells and phages injuried by ultraviolet radiation in the ecological long-wave band].

作者信息

Samoĭlova K A, Janovská E, Vizdalova M

出版信息

Tsitologiia. 1979 Jun;21(6):722-9.

PMID:462548
Abstract

Photoreactivation (PR) was measured after inactivation by far (254 nm), middle (300-315 nm) and near (315-400 nm) UV radiation of Paramecium caudatum and 8 strains of Escherichia coli differing in PR and dark repair capability. PR volume was high and practically the same after irradiation by far and middle UV, but PR was not observed in near UV-inactivated cells of all the strains. It is proposed that pyrimidine dimers are not significant in near UV lethal lesions in cells, as near UV-irradiated phages (T7 and lambdacI 857) are not photoreactivated in undamaged host bacterial cells.

摘要

在用远紫外线(254纳米)、中紫外线(300 - 315纳米)和近紫外线(315 - 400纳米)对尾草履虫和8株在光复活和暗修复能力上存在差异的大肠杆菌进行灭活后,测定了光复活(PR)情况。远紫外线和中紫外线照射后,光复活量较高且实际上相同,但在所有菌株的近紫外线灭活细胞中均未观察到光复活现象。有人提出,嘧啶二聚体在细胞的近紫外线致死损伤中并不重要,因为近紫外线照射的噬菌体(T7和λcI 857)在未受损的宿主细菌细胞中不会发生光复活。

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