Annenkov G A, Morozov V V, Dement'eva E S, Solntsev Iu B
Tsitologiia. 1975 Sep;17(9):1098-103.
A modification is described for the method of ultramicroelectrophoretic fractioning of proteins in polyacrylamide gel in capillaries 400--100 microns in diameter. The original construction of the assembly of apparatuses is offered which can be assembled from details made in the USSR. The operation of dosed filling of capillaries is significantly facilitated. The optimal quantity of protein in one sample reaches 1.5--0.1 mkg (depending on the capillary diameter). The reproducability and sharpness of protein fractions by the offered method are not worse compared with the generally accepted method of macro-disc electrophoresis.
本文描述了一种对蛋白质在直径为400 - 100微米的毛细管中的聚丙烯酰胺凝胶中进行超微电泳分级分离方法的改进。提供了该仪器组件的原始构造,其可由苏联制造的部件组装而成。显著简化了毛细管定量填充的操作。一个样品中蛋白质的最佳量达到1.5 - 0.1微克(取决于毛细管直径)。与普遍接受的宏观圆盘电泳方法相比,所提供方法的蛋白质分级分离的重现性和清晰度并不逊色。