McTaggart C, Yovich J V, Penhale J, Raidal S L
Division of Veterinary and Biomedical Sciences, Murdoch University, Western Australia, 6150.
Res Vet Sci. 2001 Aug;71(1):73-9. doi: 10.1053/rvsc.2001.0490.
Flow cytometric assays were used to compare phagocytic and oxidative burst activity of neutrophils from healthy foals less than 7 days of age with the activity of cells from healthy adult horses. The phagocytosis of Staphylococcus aureus by foal neutrophils was less than that observed for adult neutrophils when autologous serum was used as the source of opsonins in the assay. The use of adult serum did not significantly improve the ability of foal neutrophils to attach bacteria. The oxidative burst activity of foal neutrophils was equivalent to that of adult cells. However, when serum or plasma was incorporated into the oxidative burst assay, foal neutrophils demonstrated greatly reduced autofluorescence and a suppressed response to phorbol myristate acetate (PMA), relative to that demonstrated by adult cells. These results suggest that peripheral blood neutrophils from foals have a reduced ability to phagocytose bacteria relative to that exhibited by adult horse neutrophils and that the oxidative burst activity of foal neutrophils is down-regulated in response to an unidentified serum factor(s). Such changes may contribute to the increased susceptibility of foals to septic disease.
流式细胞术检测用于比较7日龄以内健康马驹中性粒细胞的吞噬和氧化爆发活性与健康成年马细胞的活性。当在检测中使用自体血清作为调理素来源时，马驹中性粒细胞对金黄色葡萄球菌的吞噬作用低于成年中性粒细胞。使用成年血清并未显著提高马驹中性粒细胞附着细菌的能力。马驹中性粒细胞的氧化爆发活性与成年细胞相当。然而，当将血清或血浆加入氧化爆发检测中时，相对于成年细胞，马驹中性粒细胞表现出显著降低的自发荧光以及对佛波酯（PMA）的反应受到抑制。这些结果表明，与成年马中性粒细胞相比，马驹外周血中性粒细胞吞噬细菌的能力降低，并且马驹中性粒细胞的氧化爆发活性因一种未知血清因子而下调。这些变化可能导致马驹对败血症疾病的易感性增加。
