Brinkman C J, Treffers W F, Broekhuyse R M
Br J Ophthalmol. 1979 Oct;63(10):704-9. doi: 10.1136/bjo.63.10.704.
Patients suffering from various corneal diseases and waiting for keratoplasty have been immunologically investigated in order to establish sensitisation to corneal antigens. The presence of lymphocytes sensitised to the soluble from human corneas, bovine corneal epithelium, and bovine corneal stroma, which all possess common antigenicity, could be demonstrated in 30%, 50%, and 23%, respectively, of all patients. In none of these patients could a positive plasma antibody titre to human corneal antigens be detected. The results suggest the dominance of T-lymphocyte activity. No correlation was found between the degree of corneal vascularisation and the presence of sensitised lymphocytes to human corneal antigens. Arrangement of the patients according to diagnosis showed that especially those suffering from herpes simplex virus keratitis reacted positively to human corneal antigens. A possible explanation is given. Lymphocytes of controls showed no or only very low stimulation with the soluble fractions of human corneas or bovine corneal stromas. The soluble fraction of bovine corneal epithelium stimulated the lymphocytes of 6 out of 19 controls. The elimination of the donor corneal epithelium before transplantation may be beneficial in view of the involvement of histocompatibility antigens.
为确定对角膜抗原的致敏情况，对患有各种角膜疾病并等待角膜移植的患者进行了免疫学研究。在所有患者中，分别有30%、50%和23%的患者体内存在对人角膜可溶性成分、牛角膜上皮和牛角膜基质致敏的淋巴细胞，这些成分都具有共同的抗原性。在这些患者中，均未检测到针对人角膜抗原的血浆抗体滴度呈阳性。结果提示T淋巴细胞活性占主导。未发现角膜血管化程度与对人角膜抗原致敏淋巴细胞的存在之间存在相关性。根据诊断对患者进行分类显示，尤其是患有单纯疱疹病毒性角膜炎的患者对人角膜抗原反应呈阳性。对此给出了一种可能的解释。对照组的淋巴细胞对人角膜或牛角膜基质的可溶性成分无刺激反应或仅有非常低的刺激反应。牛角膜上皮的可溶性成分刺激了19名对照组中的6名的淋巴细胞。鉴于组织相容性抗原的参与，移植前去除供体角膜上皮可能是有益的。
