Brinkman C J, Oerlemans-van Zutphen M P, Broekhuyse R M
Br J Ophthalmol. 1978 Feb;62(2):100-4. doi: 10.1136/bjo.62.2.100.
Rabbits were sensitised with complete bovine corneal epithelium. The lymphocyte stimulation test was performed with the lymphocytes of these rabbits using the soluble and sonicated insoluble fraction of the corneal epithelium as the antigens. A striking difference existed in the optimal test conditions for these antigen fractions. By comparing the results of the lymphocyte stimulation test with other immunological parameters, namely, skin test reaction, antibody titre, and phytohaemagglutinin stimulation of the lymphocytes, we concluded that both antigen fractions stimulate predominantly the T-lymphocyte system, although boosting augmented the humoral immune response. Stimulation of the cultured lymphocytes by both the separate and mixed antigen fractions is evidence for the existence of crossreacting antigens between the soluble and insoluble epithelial fractions.
用完全牛角膜上皮使兔子致敏。使用角膜上皮的可溶性和经超声处理的不溶性部分作为抗原，对这些兔子的淋巴细胞进行淋巴细胞刺激试验。这些抗原部分的最佳试验条件存在显著差异。通过将淋巴细胞刺激试验的结果与其他免疫参数（即皮肤试验反应、抗体滴度和淋巴细胞的植物血凝素刺激）进行比较，我们得出结论，尽管增强作用增强了体液免疫反应，但两种抗原部分主要刺激T淋巴细胞系统。单独和混合的抗原部分对培养淋巴细胞的刺激证明了可溶性和不溶性上皮部分之间存在交叉反应抗原。
