He X, Kang A H, Stuart J M
Memphis Veterans Affairs Medical Center VAMC and the Department of Medicine, University of Tennessee, 38104, USA.
J Rheumatol. 2001 Oct;28(10):2168-75.
To determine the frequency and repertoire of CD19+ B cells capable of producing antibodies reactive to type II collagen (CII) in synovial fluid (SF) and peripheral blood (PB) of patients with rheumatoid arthritis (RA) and PB of healthy control individuals.
CD19+ B cells were isolated and activated to secrete immunoglobulins (Ig) by CD4+ T cells. Frequencies of anti-CII B cells were determined by limiting dilution analysis. The isotype and cross-reactivity of the antibodies produced were determined by ELISA.
SF and PB from 5 patients and PB from 4 healthy controls were analyzed. Anti-CII CD19+ B cells were identified in all samples tested. In the RA SF, the percentage of activated B cells reactive to human CII was significantly higher than in the PB of patients with RA (p < 0.05) or controls (p < 0.01). A majority of anti-human CII B cells from patients' SF secreted IgG isotype, whereas most anti-human CII B cells in PB of patients and controls secreted IgM. The anti-CII B cells, regardless of source, are usually reactive to both native and denatured human CII, to different types of human collagens, and to type II collagens from different species.
Anti-CII CD19+ B cells responsive to activated helper T cells are present in both patients with RA and healthy individuals. However, these B cells, especially those secreting the IgG isotype, accumulate in the inflamed joints of RA patients.
确定类风湿关节炎（RA）患者滑液（SF）和外周血（PB）以及健康对照个体PB中能够产生对II型胶原（CII）有反应性抗体的CD19⁺ B细胞的频率和库。
分离CD19⁺ B细胞，并通过CD4⁺ T细胞激活以分泌免疫球蛋白（Ig）。通过有限稀释分析确定抗CII B细胞的频率。通过酶联免疫吸附测定法确定所产生抗体的同种型和交叉反应性。
分析了5例患者的SF和PB以及4例健康对照的PB。在所有测试样本中均鉴定出抗CII CD19⁺ B细胞。在RA患者的SF中，对人CII有反应性的活化B细胞百分比显著高于RA患者的PB（p < 0.05）或对照（p < 0.01）。来自患者SF的大多数抗人CII B细胞分泌IgG同种型，而患者和对照PB中的大多数抗人CII B细胞分泌IgM。无论来源如何，抗CII B细胞通常对天然和变性的人CII、不同类型的人胶原蛋白以及来自不同物种的II型胶原蛋白均有反应。
对活化辅助性T细胞有反应的抗CII CD19⁺ B细胞在RA患者和健康个体中均存在。然而，这些B细胞，尤其是那些分泌IgG同种型的B细胞，在RA患者的炎症关节中积聚。
