Gzyl A, Augustynowicz E, van Loo I, Slusarczyk J
Department of Sera and Vaccines Evaluation, National Institute of Hygiene, Chocimska Street 24, 00-791 Warsaw, Poland.
Vaccine. 2001 Nov 12;20(3-4):299-303. doi: 10.1016/s0264-410x(01)00356-5.
Growing number of Bordetella pertussis infections in 1997-1998 in Poland overshadowed the successful national vaccination program. This situation prompted us to investigate if this shift reflects changes in the B. pertussis population. We investigated the possible divergence in genes encoding pertussis toxin subunit 1 (PtxS1) and pertactin (P.69) in B. pertussis population strains during the period of 1960-2000. The pertussis toxin and pertactin variants (ptxS1B and prn1) were found in strains used for production of the whole-cell pertussis vaccine (WCV) production in Poland. Results of the study indicate that the ptxS1A-allele replaced the vaccine variant in 69% in the 1960s, and in 100% in 1990s, and although the prn1-allele was found in all strains from the 1960s and 1970s, after 1995 was gradually replaced by prn2 and prn4 variants. Presumably, vaccination could affect the population structure of B. pertussis in Poland and resulted in antigenic shift in both genes analyzed. Our findings may have implications for the composition of polish WCV and the currently licensed acellular pertussis vaccines.
1997 - 1998年波兰百日咳博德特氏菌感染病例数不断增加，给该国成功的国家疫苗接种计划蒙上了阴影。这种情况促使我们研究这种变化是否反映了百日咳博德特氏菌群体的改变。我们调查了1960 - 2000年期间百日咳博德特氏菌群体菌株中编码百日咳毒素亚基1（PtxS1）和丝状血凝素（P.69）的基因可能存在的差异。在波兰用于生产全细胞百日咳疫苗（WCV）的菌株中发现了百日咳毒素和丝状血凝素变体（ptxS1B和prn1）。研究结果表明，ptxS1A等位基因在20世纪60年代取代了疫苗变体的比例为69%，在90年代则为100%；尽管在20世纪60年代和70年代的所有菌株中都发现了prn1等位基因，但1995年后逐渐被prn2和prn4变体取代。据推测，疫苗接种可能影响了波兰百日咳博德特氏菌的群体结构，并导致所分析的两个基因发生抗原性转变。我们的研究结果可能对波兰全细胞百日咳疫苗以及目前已获许可的无细胞百日咳疫苗的成分有影响。
