Jacquez J A
J Gen Physiol. 1975 Jan;65(1):57-83. doi: 10.1085/jgp.65.1.57.
One-way fluxes in the steady state and one-way influxes at zero intracellular concentrations were measured for alpha-aminoisobutyric acid (AIB) in Ehrlich ascites tumor cells at 32 degrees C. The one-way fulxes show trans effects in the concentration of AIB and are dependent on sodium levels. The one-way fluxes for initial influx and for the steady state were fitted with the equations derived for the frequently used two-state carrier model. Estimates of the parameters of these equations were obtained with use of nonlinear least squares. These gave relatively good fits of the flux data and the data on steady-state distribution ratios. The two-state carrier model predicted a trans inhibition of one-way influx and a trans stimulation of one-way efflux. The former phenomenon has been demonstrated for AIB transport in Ehrlich ascites cells and there is evidence, through less firm, for the latter.
在32摄氏度下，对艾氏腹水癌细胞中的α-氨基异丁酸（AIB）进行了稳态下的单向通量和细胞内浓度为零时的单向流入量测量。单向通量显示出AIB浓度的反式效应，并且依赖于钠水平。初始流入和稳态的单向通量用为常用的双态载体模型推导的方程进行拟合。使用非线性最小二乘法获得这些方程参数的估计值。这些结果对通量数据和稳态分布比数据给出了相对较好的拟合。双态载体模型预测单向流入的反式抑制和单向流出的反式刺激。前一种现象已在艾氏腹水细胞的AIB转运中得到证实，并且有证据（尽管不太确凿）支持后一种现象。
