艾氏腹水癌细胞中α-氨基异丁酸的单向通量。转运效应以及钠和钾的影响。
One-way fluxes of alpha-aminoisobutyric acid in Ehrlich ascites tumor cells. Trans effects and effects of sodium and potassium.
作者信息
Jacquez J A
出版信息
J Gen Physiol. 1975 Jan;65(1):57-83. doi: 10.1085/jgp.65.1.57.
Abstract
One-way fluxes in the steady state and one-way influxes at zero intracellular concentrations were measured for alpha-aminoisobutyric acid (AIB) in Ehrlich ascites tumor cells at 32 degrees C. The one-way fulxes show trans effects in the concentration of AIB and are dependent on sodium levels. The one-way fluxes for initial influx and for the steady state were fitted with the equations derived for the frequently used two-state carrier model. Estimates of the parameters of these equations were obtained with use of nonlinear least squares. These gave relatively good fits of the flux data and the data on steady-state distribution ratios. The two-state carrier model predicted a trans inhibition of one-way influx and a trans stimulation of one-way efflux. The former phenomenon has been demonstrated for AIB transport in Ehrlich ascites cells and there is evidence, through less firm, for the latter.
摘要
在32摄氏度下，对艾氏腹水癌细胞中的α-氨基异丁酸（AIB）进行了稳态下的单向通量和细胞内浓度为零时的单向流入量测量。单向通量显示出AIB浓度的反式效应，并且依赖于钠水平。初始流入和稳态的单向通量用为常用的双态载体模型推导的方程进行拟合。使用非线性最小二乘法获得这些方程参数的估计值。这些结果对通量数据和稳态分布比数据给出了相对较好的拟合。双态载体模型预测单向流入的反式抑制和单向流出的反式刺激。前一种现象已在艾氏腹水细胞的AIB转运中得到证实，并且有证据（尽管不太确凿）支持后一种现象。
相似文献
1
One-way fluxes of alpha-aminoisobutyric acid in Ehrlich ascites tumor cells. Trans effects and effects of sodium and potassium.艾氏腹水癌细胞中α-氨基异丁酸的单向通量。转运效应以及钠和钾的影响。
J Gen Physiol. 1975 Jan;65(1):57-83. doi: 10.1085/jgp.65.1.57.
2
A reassessment of decreased amino acid accumulation by Ehrlich ascites tumor cells in the presence of metabolic inhibitors.对艾氏腹水瘤细胞在代谢抑制剂存在情况下氨基酸积累减少的重新评估。
J Gen Physiol. 1977 Jun;69(6):681-704. doi: 10.1085/jgp.69.6.681.
3
Na+-gradient-stimulated AIB transport in membrane vesicles from Ehrlich ascites cells.艾氏腹水癌细胞膜囊泡中Na⁺梯度刺激的AIB转运
J Membr Biol. 1974;18(3-4):315-34. doi: 10.1007/BF01870120.
4
The effect of reversal on Na + and K + electrochemical potential gradients on the active transport of amino acids in Ehrlich ascites tumor cells.逆转对 Ehrlich 腹水肿瘤细胞中氨基酸主动转运时钠钾电化学势梯度的影响。
Biochim Biophys Acta. 1971 Oct 12;249(1):15-33. doi: 10.1016/0005-2736(71)90079-4.
5
Effect of H+ on the kinetics of Na+-dependent amino acid transport in Ehrlich ascites tumor cells: evidence for H+ as an alternative substrate.氢离子对艾氏腹水癌细胞中钠离子依赖性氨基酸转运动力学的影响：氢离子作为替代底物的证据。
J Cell Physiol. 1981 Dec;109(3):507-16. doi: 10.1002/jcp.1041090317.
6
Amino Acid Transport and stimulation by substrates in the absence of a Na2+ electrochemical potential gradient.在不存在Na2+电化学势梯度的情况下氨基酸的转运及底物刺激作用。
J Membr Biol. 1981;62(1-2):149-60. doi: 10.1007/BF01870207.
7
K+-induced alterations of energetics and exchange diffusion in the carrier-mediated transport of the folic acid analog, methotrexate, in Ehrlich ascites tumor cells.
Biochim Biophys Acta. 1980 Dec 2;603(1):157-70. doi: 10.1016/0005-2736(80)90398-3.
8
The Na+ gradient hypothesis in cytoplasts derived from Ehrlich ascites tumor cells.
Biochem Biophys Res Commun. 1979 Dec 28;91(4):1430-6. doi: 10.1016/0006-291x(79)91226-9.
9
Na+ and K+ electrochemical potential gradients and the transport of alpha-aminoisobutyric acid in Ehrlich ascites tumor cells.钠离子和钾离子的电化学势梯度以及α-氨基异丁酸在艾氏腹水瘤细胞中的转运
Biochim Biophys Acta. 1969;193(2):368-83. doi: 10.1016/0005-2736(69)90197-7.
10
Transport of the amino acid analog, 2-aminobicyclo(2,2,1)-heptane-2-carboxylic acid, by Ehrlich ascites tumor cells.艾氏腹水癌细胞对氨基酸类似物2-氨基双环(2,2,1)庚烷-2-羧酸的转运
Biochim Biophys Acta. 1973 Jul 6;311(3):462-75. doi: 10.1016/0005-2736(73)90326-x.
引用本文的文献
1
Membrane transport during erythroid differentiation.红细胞分化过程中的膜转运。
J Membr Biol. 1982;64(1-2):11-21. doi: 10.1007/BF01870764.
2
Local blood-to-tissue transport in Walker 256 metastatic brain tumors.Walker 256转移性脑肿瘤中的局部血液到组织的转运
J Neurooncol. 1984;2(3):205-18. doi: 10.1007/BF00253272.
3
Characteristics of alpha-aminoisobutyric acid transport in rat skeletal muscles.大鼠骨骼肌中α-氨基异丁酸转运的特征
Biochem J. 1984 Jul 1;221(1):71-9. doi: 10.1042/bj2210071.
4
Vasoactive intestinal polypeptide-induced chloride secretion by a colonic epithelial cell line. Direct participation of a basolaterally localized Na+,K+,Cl- cotransport system.血管活性肠肽诱导结肠上皮细胞系分泌氯离子。基底外侧定位的Na⁺、K⁺、Cl⁻共转运系统的直接参与。
J Clin Invest. 1985 Feb;75(2):462-71. doi: 10.1172/JCI111721.
5
Equilibrium and steady-state models of the coupling between the amino acid gradient and the sodium electrochemical gradient in mouse ascites- tumour cells.小鼠腹水肿瘤细胞中氨基酸梯度与钠电化学梯度耦合的平衡和稳态模型。
Biochem J. 1978 Sep 15;174(3):811-7. doi: 10.1042/bj1740811.
6
A reassessment of decreased amino acid accumulation by Ehrlich ascites tumor cells in the presence of metabolic inhibitors.对艾氏腹水瘤细胞在代谢抑制剂存在情况下氨基酸积累减少的重新评估。
J Gen Physiol. 1977 Jun;69(6):681-704. doi: 10.1085/jgp.69.6.681.
本文引用的文献
1
THE KINETICS OF CARRIER-MEDIATED ACTIVE TRANSPORT OF AMINO ACIDS.氨基酸载体介导的主动转运动力学
Proc Natl Acad Sci U S A. 1961 Feb;47(2):153-63. doi: 10.1073/pnas.47.2.153.
2
THE POSSIBLE ROLE OF POLYVALENT CARRIERS IN CELLULAR TRANSPORTS.多价载体在细胞运输中的可能作用。
Biochim Biophys Acta. 1965 Jan 25;94:102-13. doi: 10.1016/0926-6585(65)90013-0.
3
Carrier-amino acid stoichiometry in amino acid transport in Ehrlich ascites cells.艾氏腹水癌细胞中氨基酸转运过程中载体-氨基酸的化学计量关系。
Biochim Biophys Acta. 1963 Apr;71:15-33. doi: 10.1016/0006-3002(63)90981-8.
4
Factors controlling the uptake and retention of methionine and ethionine by Ehrlich ascites carcinoma cells.艾氏腹水癌细胞摄取和保留蛋氨酸及乙硫氨酸的控制因素。
J Biol Chem. 1961 May;236:1419-24.
5
Transport and exchange diffusion of L-tryptophan in Ehrlich cells.L-色氨酸在艾氏腹水癌细胞中的转运与交换扩散
Am J Physiol. 1961 May;200:1063-8. doi: 10.1152/ajplegacy.1961.200.5.1063.
6
Potassium and sodium movements in the Ehrlich mouse ascites tumor cell.艾氏小鼠腹水瘤细胞中的钾离子和钠离子运动
J Gen Physiol. 1958 Jan 20;41(3):565-83. doi: 10.1085/jgp.41.3.565.
7
The exchangeability of glycine accumulated by carcinoma cells.癌细胞积累的甘氨酸的可交换性。
J Biol Chem. 1957 Mar;225(1):305-15.
8
The effects of sodium ions and potassium ions on glycine uptake by mouse ascites-tumour cells in the presence and absence of selected metabolic inhibitors.在存在和不存在选定代谢抑制剂的情况下，钠离子和钾离子对小鼠腹水肿瘤细胞摄取甘氨酸的影响。
Biochem J. 1967 Jun;103(3):863-76. doi: 10.1042/bj1030863.
9
Kinetic relations of the Na-amino acid interaction at the mucosal border of intestine.肠道黏膜边界处钠-氨基酸相互作用的动力学关系。
J Gen Physiol. 1967 May;50(5):1261-86. doi: 10.1085/jgp.50.5.1261.
10
Discrimination of single transport systems. The Na plus-sensitive transport of neutral amino acids in the Ehrlich cell.单一转运系统的鉴别。艾氏腹水癌细胞中对钠离子敏感的中性氨基酸转运
J Gen Physiol. 1966 Sep;50(1):203-24. doi: 10.1085/jgp.50.1.203.
Zotero 插件