Sun-Wada G H, Murakami H, Nakai H, Wada Y, Futai M
Division of Biological Sciences, Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
Gene. 2001 Aug 22;274(1-2):93-9. doi: 10.1016/s0378-1119(01)00603-5.
The 23-kDa proteolipid subunit of mouse vacuolar-type proton-translocating ATPase (V-ATPase) was predicted to be a hydrophobic polypeptide of 205 amino acid residues with five putative transmembrane segments. It exhibits sequence similarity to Vma16p of Saccharomyces cerevisiae and vha-4 of Caenorhabdittis elegans (83 and 84%, respectively). Southern blot analysis indicated that the proteolipid is encoded by a single gene, Atp6f, in the mouse genome. Atp6f was mapped to approximately 55 cM on chromosome 4, and its genomic organization is similar to that of the human gene: 8 exons separated by 7 introns, with boundaries matching the GT-AG rule. RNA blotting demonstrated that Atp6f is transcribed as 1.0- and 1.8-kb mRNAs in multiple tissues to varying degrees. The major transcription initiation sites are at -13 and -58 bp upstream of the translation initiation codon. The epitope-tagged 23-kDa protoelipid was localized in endomembrane organelles in CHO cells, as expected for a component of a vacuolar-type proton pump.
小鼠液泡型质子转运ATP酶（V-ATPase）的23-kDa蛋白脂质亚基被预测为一种由205个氨基酸残基组成的疏水多肽，具有五个假定的跨膜区段。它与酿酒酵母的Vma16p和秀丽隐杆线虫的vha-4表现出序列相似性（分别为83%和84%）。Southern印迹分析表明，该蛋白脂质由小鼠基因组中的单个基因Atp6f编码。Atp6f被定位在4号染色体上约55 cM处，其基因组结构与人类基因相似：由7个内含子分隔的8个外显子，边界符合GT-AG规则。RNA印迹显示，Atp6f在多个组织中以不同程度转录为1.0-kb和1.8-kb的mRNA。主要转录起始位点位于翻译起始密码子上游-13和-58 bp处。如液泡型质子泵的一个组分所预期的那样，带有表位标签的23-kDa蛋白脂质定位于CHO细胞的内膜细胞器中。
