Bidon N, Brichory F, Hanash S, Bourguet P, Dazord L, Le Pennec J P
Département de Médecine Nucléaire, UPRES EA 1794, Centre Eugène Marquis, CS 44 229, 35042 Rennes Cedex, France.
Gene. 2001 Aug 22;274(1-2):253-62. doi: 10.1016/s0378-1119(01)00598-4.
Galectins are animal proteins which specifically bind beta-D-galactoside residues and their specific cellular function is not yet clearly established. However, these proteins seem to play a role in neoplastic transformations. Po66 is a murine monoclonal antibody directed against a protein from human lung carcinoma, Po66 Carbohydrate-Binding-Protein (Po66-CBP), which belongs to the galectin-8 family. Our results show that the Po66-CBP gene generates five transcripts by alternative splicing, which could give rise to five proteins: two proteins belong to the tandemly repeated galectin family and three belong to the single carbohydrate recognition domain galectins. All these proteins are encoded by a unique gene located in 1q42. Experiments carried out by reverse transcriptase-polymerase chain reaction show that the levels of expression of these five galectin-8 isoforms are variable during the culture time in SK-MES-1, a human lung squamous carcinoma cell line. Cancer Genome Anatomy Project database analysis confirms the presence of Po66-CBP in lung cancer and its absence in healthy lung.
半乳糖凝集素是一类动物蛋白，它能特异性结合β-D-半乳糖苷残基，其特定的细胞功能尚未明确。然而，这些蛋白似乎在肿瘤转化过程中发挥作用。Po66是一种鼠单克隆抗体，它针对的是一种来自人肺癌的蛋白，即Po66碳水化合物结合蛋白（Po66-CBP），该蛋白属于半乳糖凝集素-8家族。我们的研究结果表明，Po66-CBP基因通过可变剪接产生五种转录本，这可能会产生五种蛋白质：两种蛋白质属于串联重复半乳糖凝集素家族，三种属于单碳水化合物识别域半乳糖凝集素。所有这些蛋白质都由位于1q42的一个独特基因编码。通过逆转录聚合酶链反应进行的实验表明，在人肺鳞癌细胞系SK-MES-1的培养过程中，这五种半乳糖凝集素-8亚型的表达水平是可变的。癌症基因组解剖计划数据库分析证实了Po66-CBP在肺癌中的存在以及在健康肺组织中的缺失。
