Manetta T, Gay V, Spreafico G, Massaro A, Piantino P
Servizio di Immunoematologia e Medicina Trasfusionale OIRM, Torino, Italy.
Minerva Med. 2001 Oct;92(5):393-400.
One of the most common technical problems of the virological laboratories is the tests applications on the open microplates systems. Immunoenzymatic methods can present difficulties in terms of results when are apply on several instruments. We have evaluated three different new virological kits from Bio-Rad: Genscreen Plus HIV 1/2 AG-AB, Monolisa anti HCV Plus V.2 and Monolisa AgHBs Plus on the automatic system Plato 3300. The purpose of this article was to present and summarize some data on these new Bio-Rad kits designed for the screening in the Blood Banks and Virological laboratories. We followed a protocol studied for working on automatic machine with different steps using negative fresh donors sera for: - study of edge effect, - study of specificity, and using also positive high value sera and seroconversion Bio-Rad panels for: - study of contamination, - study of sensitivity. The studies showed that the Bio-Rad kits and instrument were releable, flexible, easy to use and ready for each kind of routine in Blood Banks and Virological-Microbiological centres.
病毒学实验室最常见的技术问题之一是在开放式微孔板系统上进行检测应用。免疫酶法在应用于多种仪器时，结果可能会出现问题。我们在自动系统Plato 3300上评估了伯乐公司的三种不同的新型病毒学检测试剂盒：Genscreen Plus HIV 1/2 AG-AB、Monolisa anti HCV Plus V.2和Monolisa AgHBs Plus。本文的目的是展示和总结一些关于这些为血库和病毒学实验室筛查设计的新型伯乐试剂盒的数据。我们遵循了一个为在自动机器上工作而研究的方案，使用新鲜阴性供体血清进行不同步骤的操作，用于：-边缘效应研究，-特异性研究，并且还使用高值阳性血清和血清转化伯乐检测板进行：-污染研究，-敏感性研究。研究表明，伯乐试剂盒和仪器可靠、灵活、易于使用，适用于血库以及病毒学-微生物学中心的各类常规检测。
