Hjerpe A, Reinholt F P, Engfeldt B
Calcif Tissue Int. 1979 Nov 26;29(2):169-71. doi: 10.1007/BF02408073.
Compact bone tissue chondroitin sulphate, previously considered to be pure chondroitin-4-sulphate, was isolated from three adult human femoral diaphyses and digested with chondroitinase ABC. Assaying these digests by means of high performance liquid chromatography we could demonstrate that 12 to 14% of the disaccharide residues were 6-sulphated. The 4/6-ratios were also studied in chondroitin sulphate fractions of different molecular size. Slightly increasing amounts of 6-sulphated disaccharides were found with increasing size of the chondroitin sulphates.
致密骨组织硫酸软骨素，以前被认为是纯硫酸软骨素-4-硫酸盐，从三根成人股骨干中分离出来，并用硫酸软骨素酶ABC消化。通过高效液相色谱法对这些消化产物进行分析，我们可以证明12%至14%的二糖残基是6-硫酸化的。还研究了不同分子大小的硫酸软骨素组分中的4/6比例。随着硫酸软骨素大小的增加，发现6-硫酸化二糖的量略有增加。
