Elliott R J, Gardner D L
Biochim Biophys Acta. 1977 Jul 21;498(1):349-54. doi: 10.1016/0304-4165(77)90273-2.
Connective tissue extracts containing the 4- and 6-sulphated isomers of chondroitin sulphate can be measured at the microgram level (approximately 10 microgram) using colorimetric assays. The chondroitin sulphates are depolymerised to disaccharides using chondroitin ABC lyase (EC 4.2.2.4). The 4- and 6-sulphated disaccharides after treatment with acid periodate are determined using a thiobarbituric acid procedure. An alkaline borate p-dimethylaminobenzaldehyde reaction is used to measure 6-sulphated disaccharide, the 4-sulphated disaccharide not forming a chromogen. Unsulphated disaccharide causes interference in both assays and if present is separated prior to assay using paper electrophoresis fractionation.
含有硫酸软骨素4-硫酸酯和6-硫酸酯异构体的结缔组织提取物，可使用比色法在微克水平（约10微克）进行测定。使用软骨素ABC裂解酶（EC 4.2.2.4）将硫酸软骨素解聚为二糖。用高碘酸处理后的4-硫酸酯和6-硫酸酯二糖，采用硫代巴比妥酸法进行测定。用碱性硼酸盐对二甲氨基苯甲醛反应来测定6-硫酸酯二糖，4-硫酸酯二糖不形成色原。未硫酸化的二糖在两种测定中均会产生干扰，若存在则在测定前使用纸电泳分离法进行分离。
