Bünger R, Haddy F J, Querengässer A, Gerlach E
Pflugers Arch. 1975;353(4):317-26. doi: 10.1007/BF00587028.
Hemodynamic and metabolic characteristics of an isolated guinea pig heart preparation perfused with a pyruvate fortified Krebs-Ringer-bicarbonate solution are described. The preparation is stable for more than 90 min with respect to coronary flow, heart rate, left ventricular pressure, dP/dt, oxygen consumption, and myocardial high energy phosphate levels. The changes in coronary flow induced by alterations of perfusion pressure, ischemia, and hypoxia resemble those seen under in vivo conditions. The preparation also exhibits concentration dependent and reproducible changes in coronary resistance upon administration of adenosine and papaverine. The in vivo like features of this preparation can be mainly attributed to the use of pyruvate as additional and preferentially utilized substrate. The preparation appears to be suitable for quantitative studies of myocardial metabolism and heart function as well as for investigations of the coronary system.
本文描述了用丙酮酸强化的 Krebs-Ringer-碳酸氢盐溶液灌注的离体豚鼠心脏标本的血流动力学和代谢特征。该标本在冠状动脉流量、心率、左心室压力、dP/dt、耗氧量和心肌高能磷酸盐水平方面稳定超过90分钟。灌注压力改变、缺血和缺氧引起的冠状动脉流量变化与体内条件下观察到的相似。给予腺苷和罂粟碱后，该标本的冠状动脉阻力也呈现浓度依赖性和可重复性变化。该标本的体内样特征主要归因于使用丙酮酸作为额外的且优先利用的底物。该标本似乎适用于心肌代谢和心脏功能的定量研究以及冠状动脉系统的研究。
