Stumm C, Croes A F
Arch Microbiol. 1975;102(2):117-22. doi: 10.1007/BF00428355.
Synchronous gametogenesis in the water mold Allomyces arbuscula is blocked by actinomycin D added at the onset of the process. Formation of the male gametangium can be selectively inhibited by administering actinomycin one hr after the induction of gametogenesis. The polyribosome pattern obtained after density gradient centrifugation remains virtually unchanged throughout gametogenesis until a stage immediately preceding maturation of the gametes. When ribosome from gametes and swarming zygotes are analyzed on gradients, some RNase-sensitive materials is found to band in the heavier portion of the gradient. Its presence suggests that some messenger RNA associated with ribosomes is conserved in the swarming cells. During gametogenesis RNA is de novo synthesized and becomes associated with the polyribosomes.
在水霉丛枝异水霉中，同步配子发生在该过程开始时加入放线菌素D后受到阻断。在配子发生诱导后1小时给予放线菌素，可选择性抑制雄配子囊的形成。在密度梯度离心后获得的多核糖体模式在整个配子发生过程中几乎保持不变，直到配子成熟前的一个阶段。当对配子和游动合子的核糖体进行梯度分析时，发现一些对核糖核酸酶敏感的物质在梯度较重的部分形成条带。它的存在表明，一些与核糖体相关的信使核糖核酸在游动细胞中得以保留。在配子发生过程中，核糖核酸是重新合成的，并与多核糖体结合。
