Miura E M, Binotti M A, de Camargo D S, Mizubuti I Y, Ida E I
Departamento de Tecnologia de Alimentos e Medicamentos, Centro de Ciências Agrárias da Universidade Estadual de Londrina.
Arch Latinoam Nutr. 2001 Jun;51(2):195-8.
The soybean cultivar BR 36 with conventional levels of trypsin inhibitors activity and the soybean line BRM95-5262, which was genetically selected to contain low activity of trypsin inhibitors were used for biological assays with rats. BR 36 and BRM95-5262 contained 40 and 20, and 30 and 20% of relative residual activity of trypsin inhibitors, respectively. The mean values of PER and NPR showed that treatments with crude soybeans were minor than treatments with soybean thermically processed. However, the treatments of thermically processed soybean did not showed significative differences (p > or = 0.05). When the trypsin inhibitors activity were 8.61 and 8.44 UIT/mg of samples or 20 and 30% of relative residual activity of cultivar BR 36 and line BRM95-5262, respectively, it was observed that mean values of PER and NPR were not significatives. The mean values of CDA and CDV of treatments with crude soybeans were minor than treatment with casein and similar to the treatments with soybean thermically processed. So, it can be concluded that the biological evaluation obtained with soybean protein were dependent of initial trypsin inhibitors activities and of its respective thermical treatment. There was advantage in the use of BRM95-5262 soybean line with low trypsin inhibitors activity.
将具有常规水平胰蛋白酶抑制剂活性的大豆品种BR 36和经基因选育以含有低活性胰蛋白酶抑制剂的大豆品系BRM95 - 5262用于大鼠的生物学试验。BR 36和BRM95 - 5262的胰蛋白酶抑制剂相对残余活性分别为40%和20%,以及30%和20%。蛋白质效率比值(PER)和净蛋白质比值(NPR)的平均值表明,用粗大豆进行的处理低于用热处理大豆进行的处理。然而,热处理大豆的处理之间没有显著差异(p≥0.05)。当胰蛋白酶抑制剂活性分别为8.61和8.44国际单位/毫克样品,即品种BR 36和品系BRM95 - 5262的相对残余活性分别为20%和30%时,观察到PER和NPR的平均值没有显著差异。用粗大豆进行处理的蛋白质消化率校正氨基酸评分(CDA)和蛋白质消化率校正生物价(CDV)的平均值低于用酪蛋白进行的处理,且与用热处理大豆进行的处理相似。因此,可以得出结论,用大豆蛋白获得的生物学评价取决于初始胰蛋白酶抑制剂活性及其各自的热处理。使用具有低胰蛋白酶抑制剂活性的BRM95 - 5262大豆品系具有优势。