Sandholm M, Smith R R, Shih J C, Scott M L
J Nutr. 1976 Jun;106(6):761-6. doi: 10.1093/jn/106.6.761.
A new method is described for determination of antitrypsin activity based on inhibition of trypsin solubilization of calcium caseinate in agar plates. The method was applied to analyze soybeans after graded heat treatments for their antitrypsin content. Biological determination of protein and energy values of the soybean samples showed direct correlation of these values with the destruction of antitrypsin as measured by the new method, using rainbow trout.
本文描述了一种基于抑制胰蛋白酶对琼脂平板中酪蛋白钙的溶解作用来测定抗胰蛋白酶活性的新方法。该方法用于分析经过分级热处理的大豆的抗胰蛋白酶含量。利用虹鳟鱼对大豆样品的蛋白质和能量值进行生物学测定，结果表明这些值与用新方法测定的抗胰蛋白酶的破坏程度直接相关。
