美洲叶甲（鞘翅目，叶甲科）卫星DNA的特征及染色体定位

Characterization and chromosome location of satellite DNA in the leaf beetle Chrysolina americana (Coleoptera, Chrysomelidae).

作者信息

Lorite P, Palomeque T, Garnería I, Petitpierre E

机构信息

Area de Genética, Departmento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Spain.

出版信息

Genetica. 2000;110(2):143-50. doi: 10.1023/a:1017926127213.

DOI:10.1023/a:1017926127213

PMID:11678504

Abstract

This paper is the first record of the satellite DNA of the specialized phytophagous genus Chrysolina. The satellite DNA of Chrysolina americana is organized in a tandem repeat of monomers 189 bp long, has a A + T content of 59.6% and presents direct and inverted internal repeats. Restriction analysis of the total DNA with methylation sensitive enzymes suggests that this repetitive DNA is undermethylated. In siti hybridization with a biotinylated probe of the satellite DNA showed the pericentromeric localization of these sequences in all meiotic bivalents. The presence of this repetitive DNA in other species of the genus was also tested by Southern analysis. The results showed that this satellite DNA sequence is specific to the C. americana genome and has not been found in three other species of Chrysolina with a different choice of host plants than in the former.

摘要

本文是关于专食性叶甲属Chrysolina卫星DNA的首次记录。美洲叶甲（Chrysolina americana）的卫星DNA由189 bp长的单体串联重复组成，A + T含量为59.6%，并存在正向和反向内部重复序列。用甲基化敏感酶对总DNA进行限制性分析表明，这种重复DNA甲基化不足。用卫星DNA的生物素化探针进行原位杂交显示，这些序列在所有减数分裂二价体的着丝粒周围定位。还通过Southern分析检测了该属其他物种中这种重复DNA的存在情况。结果表明，这种卫星DNA序列是美洲叶甲基因组特有的，在另外三种寄主植物选择与前者不同的Chrysolina物种中未发现。