Woehler M E, Chism G E, Cox W R, Lindeman J G, Lovins R E
J Immunol Methods. 1975 Jan;6(3):301-4. doi: 10.1016/0022-1759(75)90073-3.
An immunoabsorbent column employing a DNP derivative having restricted molecular freedom (dinitrophenylated-para-aminobenzoylglutamate--Sepharose 4B) was prepared in order to isolate and purify DNP specific antibodies in high yield. The antibodies were recovered from the immunoabsorbent column in yields from 80 to 90% using 3 M sodium thiocyanate, and these antibodies retained their immunologic activity. There appeared to be limited selection of antibodies with specific affinities as was noted in a parallel experiment comparing a second antibody purification procedure.
