Sepharose gels as antigenic matrices for specific binding of anti-galactosyl antibodies.

From 100 ml of pooled normal serum taken from different species (man, cow, rabbit, chicken, duck, carp) 0.5 to 4.0 mg of high and low molecular weight anti-galactosyl antibodies could be isolated by affinity-chromatography on non-ligand-attached Sepharose 4B. The antibodies bound to the agarose matrix were eluted with 0.5 M D-galactose containing buffers. Using species-specific anti-immunoglobulin sera in immunoelectrophoresis, the immunoglobulin character of the protein fractions prepared was analyzed. In addition, the H and L chain structure of the anti-galactosyl antibodies was checked by SDS-PAGE.