Cantoni G, Padula P, Calderón G, Mills J, Herrero E, Sandoval P, Martinez V, Pini N, Larrieu E
Secretaría de Estado de Salud, Provincia de Río Negro, Argentina.
Trop Med Int Health. 2001 Oct;6(10):811-6. doi: 10.1046/j.1365-3156.2001.00788.x.
We conducted a small mammal trapping study to investigate temporal variation in prevalence of infection in hantavirus reservoir populations in the Patagonian Andes mountain range, Rio Negro province, Argentina. Rodent blood samples collected in natural and periurban habitats and at the home of an hantavirus pulmonary syndrome (HPS) case patient were analysed by enzyme-linked immunosorbent assay. Organ tissue samples were tested by polymerase chain reaction (PCR) and nucleotide sequence analysis. Eight species of 1032 rodents were captured in 15 551 trap nights, giving an overall trap success of 6.6%. Hantavirus antibody was detected in 30 of 555 Oligoryzomys longicaudatus (reservoir of Andes virus), three of 411 Abrothrix longipilis, and one of 10 Loxodontomys micropus. Antibody prevalences in O. longicaudatus were 13.7% in spring 1996, 59.3% in summer 1996, 2.1% in autumn 1997, 12.4% in winter 1997 and 3.1% in spring 1997. A much higher antibody prevalence (33%) was found during trapping around the residence of an HPS case patient. Higher prevalences were found in older male O. longicaudatus. There was no apparent correlation of antibody prevalence with rodent population density, or of rodent population density or antibody prevalence with numbers of human cases. For an HPS case that occurred in our study area in 1997, we identified the probable rodent reservoir and likely site of exposure by matching the genetic sequences of virus obtained from a rodent and the HPS case patient.
我们开展了一项小型哺乳动物诱捕研究，以调查阿根廷内乌肯省巴塔哥尼亚安第斯山脉汉坦病毒宿主种群感染率的时间变化。通过酶联免疫吸附测定法分析了在自然和城郊栖息地以及一名汉坦病毒肺综合征（HPS）病例患者家中采集的啮齿动物血液样本。通过聚合酶链反应（PCR）和核苷酸序列分析对器官组织样本进行检测。在15551个诱捕夜中捕获了1032只啮齿动物，分属8个物种，总体诱捕成功率为6.6%。在555只长尾稻鼠（安第斯病毒宿主）中有30只检测到汉坦病毒抗体，在411只长须鼠中有3只，在10只小驼鼠中有1只。长尾稻鼠的抗体感染率在1996年春季为13.7%，1996年夏季为59.3%，1997年秋季为2.1%，1997年冬季为12.4%，1997年春季为3.1%。在一名HPS病例患者住所周围进行诱捕时发现抗体感染率要高得多（33%）。在年龄较大的雄性长尾稻鼠中发现了更高的感染率。抗体感染率与啮齿动物种群密度之间没有明显相关性，啮齿动物种群密度或抗体感染率与人类病例数之间也没有明显相关性。对于1997年在我们研究区域发生的一例HPS病例，我们通过匹配从一只啮齿动物和该HPS病例患者身上获得的病毒基因序列，确定了可能的啮齿动物宿主和可能的暴露地点。
